Genomic Medicine, Biomarkers, and Health Sciences
Volume 3, Issue 1 , Pages 9-16, March 2011

Molecular Detection of Circulating Tumor Cells With Multiple mRNA Markers by Genechip for Colorectal Cancer Early Diagnosis and Prognosis Prediction

  • Shiu-Ru Lin

      Affiliations

    • School of Medical and Health Sciences, Fooyin University, Kaohsiung, Taiwan
    • Department of Medical Research, Fooyin University Hospital, Ping-Tung, Taiwan
    • Corresponding Author InformationCorresponding author. School of Medical and Health Sciences, Fooyin University, 151 Jinsyue Road, Daliao District, Kaohsiung 831, Taiwan
    • ,
  • Ming-Yii Huang

      Affiliations

    • Department of Radiation Oncology, Cancer Center, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan
    • Department of Radiation Oncology, Faculty of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan
    • ,
  • Hui-Jen Chang

      Affiliations

    • School of Medical and Health Sciences, Fooyin University, Kaohsiung, Taiwan
    • Department of Medical Research, Fooyin University Hospital, Ping-Tung, Taiwan

Received 7 January 2011; received in revised form 10 January 2011; accepted 10 January 2011.

Article Outline

Early detection is the hallmark of successful cancer treatment. Evidence is accumulating that primary cancers begin shedding neoplastic cells into the circulation at an early stage. To date, many different methodologies have been used for the detection of circulating tumor cells (CTCs) with variable sensitivity and specificity. In many studies, including patients with different clinical stages of colorectal cancer, the detection of CTCs in early and/or metastatic colorectal cancer has been shown to correlate with unfavorable clinical outcome. However, a high-sensitivity and high-throughput method for the detection of CTCs is currently lacking. Here, we introduce a high-sensitivity genechip analysis method from a colorimetric membrane array to a weighted enzymatic chip array in order to detect the CTC-related multiple mRNA markers derived from colorectal cancer patients as a convenient and practical tool for early diagnosis and prognosis prediction.

Key Words:  circulating tumor cells , colorectal cancer , colorimetric membrane array , early detection , prognosis prediction , weighted enzymatic chip array

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PII: S2211-4254(11)60003-4

doi:10.1016/S2211-4254(11)60003-4

Genomic Medicine, Biomarkers, and Health Sciences
Volume 3, Issue 1 , Pages 9-16, March 2011

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